Aromatase, also called estrogen synthase, is an enzyme responsible for a key step in the biosynthesis of estrogens. Some breast cancers require estrogens for growth and if deprived of these hormones, will regress. Consequently, oestrogen deprivation therapy is a major treatment strategy for hormone-dependent breast cancer. There are various forms of endocrine therapy but recently agents inhibiting the aromatase enzyme which catalyzes the conversion of androgens to estrogen, have been increasingly used with some degree of success. 
With the clinical success of several synthetic aromatase inhibitors (AIs) in the treatment of postmenopausal estrogen receptor-positive breast cancer, researchers have also been investigating the potential of natural products as AIs. Natural products from terrestrial and marine organisms provide a chemically diverse array of compounds not always available through current synthetic chemistry techniques. Natural products that have been used traditionally for nutritional or medicinal purposes with reduced side effects. 
P53 – Tumor Supressor and the ‘Gatekeeper’ Gene
Both the functional loss of p53 tumor supresser and the over-expression of aromatase are important for the progression of breast cancer in postmenopausal women.  Additionally a mutation in TP53 at an early stage of cancer progression can occur due to exposure to a carcinogen, virus or fungus.
Studies have shown that potent antioxidants can be found as natural compounds in healthy food sources and can protect and restore the p53 gene following damage. seeks to deliver potent yet safe natural plant botanicals via tincture directly into the bloodstream providing the body with many tools to combat excess estrogenic expression, fungal overload and other toxicities. The following table gives an at-a-glance image of the actions of the individual botanicals within
is not intended for use for children or during pregnancy or when breast feeding or for those who may have allergies to chamomile and dandelion and should be used under the care of a licensed medical practitioner. Andrographis/Artemisinin are natural contraceptives and may cause miscarriage.
Why use a Tincture?
Because a tincture is taken ‘sublingually’ directly under the tongue, the plant constituents enter the bloodstream in their pure form much more directly than by any other means, this is because they avoid being metabolised by the liver and changed, which makes them far more potent, subject to dosage and regularity. This means that the action in the body is usually quicker. Although some herbs will have an immediate effect, such as those used to help one relax. Others that are more nutritive and building in nature.
Nutritive tinctures may take several weeks of continual use before best results are seen. Tinctures are also very convenient, as nothing needs to be brewed. You simply take the drops of tincture and you’re done. You can easily carry a bottle of tincture in your bag/coat and have it available to you at all times.
Are Tinctures Safe for Pregnant or Nursing Women?
Yes, normally tinctures are fine for pregnant and nursing women, even the alcohol-based versions, however, contains some herbs which are natural contraceptives, so must NOT be used by pregnant or nursing mothers. One dose of an alcohol-based tincture has approximately the same alcohol content as eating a very ripe banana, if the tincture is too hot when held under the tongue, dilute is with a small sip of clean water.
contains some of the most potent and safe botanicals known to have antitumor, antiviral, antifungal, anti-estrogenic aswell as blood, dna and bone supporting qualities, whereas synthetic drug-based AI’s are known to cause bone loss (bone resorption caused by increased osteoclasts) possibly leading to osteoporosis. In postmenopausal women, synthetic aromatase inhibitors increase bone loss at an average rate of 1 to 3% per year.
In young women who have had ovarian suppression followed by synthetic aromatase inhibitor therapy, bone density is lost at an average of 7 to 8% per year, however natural AI’s may actually have a bone protective role, please read on.
Protection against Estrogen Deficient Bone Resorption
contains four herbs which contain contain flavonoids which have been shown to significantly improve bone formation and bone strength in animal studies, the main compounds found to have the strongest bone supportive effects include Green Tea, Nettle, Milk Thistle, Dandelion, Red Clover, Olive Oil, Cat’s Claw and Cyanidin-3-glucoside from Purple Corn Seeds. Flavonoids are a large group of naturally occurring compounds (many are found in foods), and are classified by structure into several groups including flavonols and isoflavones.
The most common type of osteoporosis is bone loss associated with ovarian hormone deficiency at menopause. There is evidence that diets which contain high levels of phytoestrogenic isoflavones are associated with a low incidence of osteoporosis and menopausal symptoms. A study in TNF-a and IL-1 must be inhibited simultaneously if bone loss is to be prevented 
Several years ago, German researchers discovered that a traditional European herbal remedy for rheumatism, (urticae dioicae foliorum) nettle leaf extract , inhibits TNF-alpha and IL-1 beta. Nettle “may inhibit the inflammatory cascade in autoimmune diseases and rheumatoid arthritis,” concluded a team of researchers. 
One of the ways Nettle leaf extract blocks proinflammatory signaling by inhibiting the genetic transcription factor that activates TNF-alpha and IL-1 beta in synovial tissue. This proinflammatory transcription factor, known as nuclear factor kappa beta (NF-kb), is elevated in chronic inflammatory diseases and is essential to activation of TNF-alpha. Nettle is thought to work by preventing degradation of the natural inhibitor of NF-kb in the body. TNF-alpha also activates NF-kb in synovial cells, leading to the suggestion that a cycle of cross-activation between TNF-alpha and NF-kb and may sustain and amplify the disease process in rheumatoid arthritis.  Cat’s Claw also acts as a potent TNF-alpha inhibitor through NF-kappaB.
So in order to address the concerns and mitigate the possibilities of bone formation loss we have included nettle leaf and other botanicals which are AI’s and also play a role in bone health; cat’s claw, red clover, dandelion and milk thistle extracts in the tincture.
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Andrographis (AT, AV)
Angrographis exerts direct anticancer activity on cancer cells by cell-cycle arrest. Immunostimulatory activity of andrographolide is evidenced by increased proliferation of lymphocytes and production of interleukin-2. Angrographis also enhances the tumor necrosis factor-alpha production resulting in increased cytotoxic activity of lymphocytes against cancer cells. 
Andrographia paniculata a Miracle Herbs for cancer treatment:In vivo and in vitro studies against Aflatoxin B1 Toxicity
Artemisinin reveals profound activity not only against malaria, but also against cancer in vivo and in clinical trials.  Artemisinin is a chemical compound that reacts with iron to form free radicals which can kill cells. By taking artemisinin with iron such as with nettle, artemisinin could be selectively picked up and concentrated by cancer cells. 
Astragalus (AV, I)
The antiviral activity of Astragalus is most likely to be due to increased immunity and possibly enhanced interferon production. Astragalus demonstrates slight inhibitory activity against adenovirus type 7 in vitro. Saponins from Astragalus stimulate the natural killer cell activity of human peripheral blood lymphocytes and restore steroid-inhibited natural killer cell activity. [15, 26]
The plant’s healing properties come from its daisy-like flowers, which contain flavonoids (apigenin) and other therapeutic substances.
Chamomile has been used for centuries in teas as a mild, relaxing sleep aid, treatment for fevers, colds, stomach ailments, and as an anti-inflammatory, to name only a few therapeutic uses. Chamomile’s therapeutic activity, including antipeptic, antispasmodic, antipyretic, antibacterial, antifungal, and anti-allergenic activity.
Cat’s Claw(AI, AT, B, I)
Cat’s Claw is a medicinal herb that has been used in the treatment of different diseases including cancer. Cat’s Claw used with chemotherapy and radiation, reported fewer adverse effects to those therapies.  Cat’s Claw helps in the restoration of cellular DNA.  It modulates the activity in the immune system, such as the proliferation of normal T and B lymphocytes , also modulating certain cytokines, including IL-1 and IL-6, TNF-a. [5, 10, 12]
Red Clover (AI, B)
Randomized, double-blind, placebo-controlled clinical trials have found that the flavonoids in Red Clover help keep bones strong and healthy in pre and peri-menopausal women. Flavonoids may do this by supporting normal bone remodeling process. 
Red clover contain isoflavones, which have a high affinity to estrogen, progesterone and androgen receptors and may reduce risk of breast cancer.  Red clover may also block the enzyme 5-alpha-reductase thought to contribute to prostate cancer in men. 
Nettle (AT, B)
Tumor necrosis factor alpha (TNF-a) is a dangerous chemical messenger (cytokine) that incites the immune system to attack healthy tissues throughout the body. Elevated TNF-a causes a systemic inflammation that results in age related neurological & vascular problems and the catabolic wasting effect seen in cancer. Nettle is thought to work by preventing degradation of the natural inhibitor of NF-kb & TNF-a levels in the body.[21, 23] The iron high content is also synergistic with Artemisinin.
Milk Thistle (L, B)
Milk thistle detoxifies synthetic chemicals that find their way into our bodies, from alcohol to heavy metals and radiation. The active ingredient, compound in milk thistle is known as silymarin which actually consists of a group of compounds called flavonolignans, helps repair damaged liver cells by stimulating protein synthesis. Silymarin also encourages liver cell growth, reduces inflammation and has potent antioxidant and anti-cancer effects. 
Dandelion (L, B)
Dandelion extracts reduce levels of reactive oxygen species (ROS) and nitric oxide production (NO) and inhibit COX-2 expression, thus making it anti- carcinogenic, but also anti-inflammatory, and antiangiogenic.  If we can stop cancers from growing blood vessels we can slow the growth of the cancer or sometimes shrink it. Dandelion roots tested in the lab on leukemia cells from blood have been known to cause apoptosis while leaving healthy cells intact.  Dandelion is known as a liver tonic, digestive stimulant, and diuretic and is also a blood purifier.
Lycium (Goji Berries)(AI)
Selective estrogen receptor modulators represent accepted therapy for estrogen receptor positive (ER+) breast cancer treatment, but have adverse side effects.  In a study in 2015, Lycium demonstrated an increase in pro-apoptotic and a decrease in anti-apoptotic proteins’ expression in cells treated with the extract. Anticancer activity and lack of toxicity against normal cells indicate a chemopreventive potential of Goji berries in breast cancer treatment.
Amla – Gooseberry (AT, AV)
In studies Amla was found induced AP-1 inhibition and was accompanied by suppression of viral transcription that resulted in growth inhibition of cervical cancer cells. AP-1 is cell controller for proliferation, and apoptosis. Growth inhibitory activity of Amla was primarily manifested through induction of apoptotic cell death. Results suggest that Amla exhibits its anti-cancer activities through inhibition of AP-1 and targets transcription of viral oncogenes responsible progression of cervical cancer. [19, 20]
Key: (L)Liver Support, (AI)Aromatase Inhibitor, (B)Bone Support, (AV)Antiviral, (AT)Antitumour, (I)Immune Support.
The following studies pertain to the references in brackets [, ] on the preceding pages.
1. Natural Products as Aromatase Inhibitors
2. lemur tyrosine kinase-3 (LMTK3) expression in breast cancer: association with tumor phenotype and clinical outcome. http://www.ncbi.nlm.nih.gov/pubmed/21671015
3. Effects of phytoestrogenic isoflavones from red clover (Trifolium pratense L.) on experimental osteoporosis.
4. Chamomile: A herbal medicine of the past with bright future
5. Uncaria tomentosa acts as a potent TNF-alpha inhibitor through NF-kappaB.
6. The antiproliferative effects of Uncaria tomentosa extracts and fractions on the growth of breast cancer cell line.
7. DNA repair enhancement of aqueous extracts of Cat’s Claw in a human volunteer study.
http://www.ncbi.nlm.nih.gov/pubmed/11515717 ( CMED100 )
8. Multitargeted therapy of cancer by silymarin
9. The efficacy of dandelion root extract in inducing apoptosis in drug-resistant human melanoma cells.
10. Treatment of THP-1 cells with Uncaria tomentosa extracts differentially regulates the expression if IL-1beta and TNF-alpha.
11. Pentacyclic oxindole alkaloids from Cat’s Claw (Uncaria tomentosa) induce human endothelial cells to release a lymphocyte-proliferation-regulating factor.
12. Antioxidant activity of ethanolic and aqueous extracts of Uncaria tomentosa (Willd.) DC
13. Activity of Artemisia annua and artemisinin derivatives, in prostate carcinoma.
14. Targeted treatment of cancer with artemisinin and artemisinin-tagged iron-carrying compounds.
15. Astragaloside IV inhibits adenovirus replication and apoptosis in A549 cells in vitro.
Lycium barbarum inhibits growth of estrogen receptor positive human breast cancer cells by favorably altering estradiol metabolism.
17. Anticancer effect of ethanol Lycium barbarum (Goji berry) extract on human breast cancer T47D cell lines
18. Enhanced expression of aromatase in p53-inactivated mammary epithelial cells.
19. Antitumour effects of Phyllanthus emblica L.: induction of cancer cell apoptosis and inhibition of in vivo tumour promotion and in vitro invasion of human cancer cells.
20. Amla (Emblica officinalis Gaertn), a wonder berry in the treatment and prevention of cancer.
Effects of Nettle (urtica dioica) extract on experimental acute pancreatitis model in rats
21. Complementary Medicine for Prostate Cancer: Effects of Soy and Fat Consumption
22. Phytoestrogens derived from red clover: an alternative to estrogen replacement therapy?
23. Antioxidant and apoptotic effects of an aqueous extract of Urtica dioica on the MCF-7 human breast cancer cell line
24. Efficient induction of extrinsic cell death by dandelion root extract in human chronic myelomonocytic leukemia (CMML) cells.
25.Inhibitory effects of neoandrographolide on nitric oxide and prostaglandin E2 production in LPS-stimulated murine macrophage.
26. The two isomers of HDTIC compounds from Astragali Radix slow down telomere shortening rate via attenuating oxidative stress and increasing DNA repair ability in human fetal lung diploid fibroblast cells.
27. A study in TNF-a and IL-1 must be inhibited simultaneously if bone loss is to be prevented.
28. Ex-vivo in-vitro inhibition of lipopolysaccharide stimulated TNF-alpha and IL-1 beta secretion in human whole blood by Nettle (urticae dioicae).
29. Antiosteoclastic activity of milk thistle extract after ovariectomy to suppress estrogen deficiency-induced osteoporosis.
30. Artemisinins and immune system.
herbal tincture is typically taken as a 10-20 drops held under the tongue for 2 mins or in a glass of warm distilled water to remove the alcohol if desired. A standard suggested adult dosage for tinctures is 1 droppersful three times a day two droppersful if adding to water as a tea.
To take a tincture, it is best to take the drops directly under the tongue. This gets the herbs directly into the bloodstream. If necessary, it is fine to dilute the tincture in a small amount of water or juice. It may be flavored with lemon or honey to disguise the taste, but actually, bitterness is a valuable effect for the body. (Note: never give honey to a child under one year of age.) You may also put the droppersful of tincture into a cup of warm or hot water for an instant cup of herbal tea. Heat your water first, before adding the herbs. Avoid heating the herbs in a microwave, they may kill or weaken their healthful benefits. Taking the tincture directly under the tongue and avoiding any liquids or foods for at least 15 minutes afterwards provides the best results.
For highest potency:
Take 1 squirt / 5 times a day (every 3 hrs) and hold under the tongue for 2-10 mins or longer. After at least 2 mins, add a little water into the mouth and gargle with it for 10-15 seconds at the back of the throat by tilting your head back before swallowing. This makes use of additional absorbtion via the tonsillar ring at the back of the throat and then delivers to the stomach where other important absorptions can take place, especially for cat’s claw.
There are 1500 drops in 50ml.
1 squirt of the dropper is approx 15-20 drops = o.66ml
How long will the 50ml bottle last?
3 squirts/day = 25 days, 4 squirts/day = 18 days , 5 squirts/day = 15 days
might best be used as part of the full RTKAI protocol* which includes the addition of ButeinAI, Extra Virgin Olive Oil, Purple Corn Extract and decaf Green Tea Extract, Curcumin with Boswellia, liver & colon detoxing, parasite cleansing, probiotics, enzymes and excercise.
These statements have not been evaluated by the Food & Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.